Cross-talk Calibrating Based on Correlation Analysis in High-throughput Genome Sequencing

摘要：

The images, in high-throughput genome sequencing of sequencing by synthesize, consist of A, C, G, T base fluorescence images, and the fluorophore emission spectra partially overlap, which causes cross-talk problem. This paper presents a method of correlation analysis, construct the calibration matrix to eliminate interference of the fluorophore. The experiment result shows the method provided in this article is effective to correct cross-talk influence, and get the deconvolution of the clusters signal.

作者: Bing-gang Ye Xiao-ming Wu Li Jing-xiang Zhou Yan Wang De-peng

作者单位: School of Bioscience and Bioengineering, South China University of Technology Guangzhou, China Shenzhen BGI Shenzhen, China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）