Cellular Biological Influences of Down-regulated MicroRNA-143 on Human Bronchial Epithelial Cells
MicroRNAs (miRNAs) were recently identified to play crucial roles in tumorigenesis processes. In present study, we explored the potential influence of miRNA-143 on several cellular biological processes related with lung cancer development in human bronchial epithelial cells 16-HBE. Chemosynthesis anti-miR-143 was used to down-regulate expression of miR-143. Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay after 24, 48 and 72 h transfection of anti-miR-143. Apoptosis and cell cycle were detected by flow cytometry after 48 h transfection of anti-miR-143. The results revealed that there were no significant differences in cell activity and apoptosis between transfection group and control group (P>0.05). Compared with control group, DNA content of G1 period of anti-miRNA-143 group had a mild decrease (P＜0.05), DNA content of S period of anti-miRNA-143 group had a mild increase (P＜0.05). The results suggested that down-regulated miR-143 induce S arrest of cell cycle. MiR-143 may associate with lung cancer development through influence cell cycle progression. The cooperation between more miRNAs and environmental carcinogen on the tumorigenesis of lung cancer needs to further investigate.
Ge Yu Liang Xi Kai Wang Yan Qiu Zhang Li Hong Yin Yue Pu Pu
Key Laboratory of Environmental Medicine and Engineering, Ministry of Education; School of Public Health, Southeast University, SEU, Nanjing, 210009, China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）