Survival of Lactobacillus acidophilus encapsulated as freeze-dried culture under extreme conditions
Lactobacillus acidophilus La-1 was encapsulated as freeze-dried culture and its survival rates under various extreme conditions were investigated in this work. The survival rate of the freeze-dried bacteria remained at 87.1% with the viable counts of 2.26×108 cfu/mL, while keeping at pH 2.5 for 2 h, and the micronized culture was also tolerant to 6% NaCl. In 0.1% bile salt medium, the viable counts of Lactobacillus acidophilus La-1 reached to 3.39×109 cfu/mL, and it could also grow exposing to 0.5% bile salt medium. The viable counts were 9.33×107 and 1.03×108 cfu/mL, with survival rates of 44.64% and 49.28%, after keeping in simulated gastric or intestinal juice for 2 h, respectively. These results indicated that the encapsulation of Lactobacillus acidophilus La-1 as lyophilized culture could be quite effective for the bacteria to survive in extreme conditions, and the freeze-dried culture powder might be thus appropriate to be applied in foods or nutritious supplements.
Chang-lu Wang Da-wei Liu Yu-rong Wang Feng-juan Li Mian-hua Chen Yan-yan Ma
Key Laboratory of Food Nutrition and Safety, Ministry of Education College of Food Engineering and B Key Laboratory of Food Nutrition and Safety, Ministry of Education College of Food Engineering and B
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）