Subcellular localization of GFP-Tetherin fusion protein in COS-1 by Confocal Laser Scanning Microscope

摘要：

tetherin cDNA was amplified from total RNA prepared from HeLa cells by RT-PCR, then cloned into the eukaryotic expression vector pEGFP-C3. The generated pEGFP-Tetherin construct was transfected into COS-1 to determine the expression and the subcellular localization of GFP-Tetherin fusion protein. Under CLSM the localization of GFP-Tetherin fusion protein distributed outside the nucleus and co-localized with TGN in COS-1 cells.

作者: Yang Yishu Lin Xiaoyan Sun Xiaona Wang Xiaoli Wu Senren Xiao Xiangqian Li Zelin Zeng Yi

作者单位: College of L ife Science and Bioengineering Beijing University of Technology Beijing China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-3

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）