Sequence Characterization and Molecular Detection of the 3′-terminal Region of Bean yellow mosaic virus Infecting Canna
The first sequence obtained from a Potyvirus infecting Canna plants (Canna spp.) revealed that it is a new strain of Bean yellow mosaic virus (BYMV), without noticeable recombination with other plant RNA viruses from this region being found. Using a degenerate primer for all Potyvirus, a DNA fragment of about 1.6kb size was amplified from total RNA samples extracted from natural-diseased Canna plants. Results of multiple alignments showed that CP nucleotide sequence, its deduced amino acid sequence and 3′ UTR sequence of BYMV Canna isolate (BYMVHZCanna) had homologies of 81.5%~85.8%, 84.7%~91.6% and 93.0%~95.9% to those of other isolates recorded by GenBank. BYMVHZCanna alone was classified in a cluster in three phylogenetic trees, which were based on CP nucleotide sequences, its deduced amino acid sequences and 3′ UTR sequences of all BYMV isolates. Field survey with RT-PCR detection showed that this virus has been widely spread in all the surveyed areas such as Hangzhou, Guangzhou and Shenzhen cities, in China.
ZuoDong QIN QianZhuo MAO AiPing DING LeiLei JIN WenJun Peng ZhiYou DU SuSu SHENTU JiShuang CHEN
Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou, China Shenzhen Institute of Landscape Gardening, Shenzhen, China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）