Cloning, expression and immunocompetence analysis of gene encoding the glutamate dehydrogenase of Streptococcus suis serotype 2
This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody. RESULTS: The fusion protein was efficiently expressed. Western blotting analysis confirmed that the resulting protein was immunogenic. CONCLUSIONS: The GDH gene of S.suis 2 was successfully cloned and the high expressed, which facilitated the further studies on the development of immunological detection and vaccine design.
Wei-Jun Yang Li Cui Xiu-Guo Hua Zhi-Biao Yang Xue-Hui Cai Yong-Qing Hao Sheng-Bo Zheng Dao-Liang Lan Jiang-Ping Gu Rui Xing Cong-Li Yuan
Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jia Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jia Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin V College of Animal science and Animal medicine, Inner Mongolia Agriculture University, Huhhot 010018,
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）