Optimization of liquid culture conditions of protease from Mucor by Box-Behnken design

摘要：

the nutritional parameters of protease, which is isolated from mao-tofu and identified as Mucor spp, were studied by response surface methodology (RSM). Glucose, soybean protein and KH2PO4 were analyzed by Box-Behnken design for optimizing their concentrations in the medium and the effect of their mutual interaction on protease activity. Glucose and soybean protein were found in influencing the protease activity highly significant. Glucose and soybean protein also showed an effect on the protease activity in interaction with other variables. The optima values of the parameters obtained through RSM, 0.72% glucose, 2.58% soybean protein and 0.1% KH2PO4 were applied. Actual value of verifiable experimentation was that the protease activity was 184.42±0.54 U/L at the optima conditions.

作者: Zhang Na Guo Qing Qi Zhao Xin Huai

作者单位: College of Food Engineering Harbin University of Commerce Harbin, China College of Forestry Northeast Forestry University Harbin, China Laboratory of Dairy Science of Ministry of Education Northeast Agricultural University Harbin, China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）