Prevent Mammary Cancer Cell Adhesion in Microvasculature in vivo by Mild Hypothermia
To investigate the effect of mild hypothermia on the mammary cancer cell adhesion to intact microvessels wall, video microscopy was used to measure the adhesion rates of mouse normal mammary epithelial cells (Nmumg) and wild type (WT) in the post-capillary venules of rat mesentery in vivo. Individual post-capillary venule was cannulated and perfused with cells (~4 million/ml) at a rate of ~1 mm/s, 1) under normothermia, 2) after pretreatment of the microvessel with mild hypothermia. Our results showed that in ~60min, a) normal cells adhere poorly to microvessels in vivo while WT adhere to a much larger extent as compared to normal cells; b) in 60 min, mild hypothermia treatment decreased the number of adherent WT tumor cells from 9.9 (±1.0 SE, n=7) to 6.6 (±0.8 SE, n=6) cells/(5000 μm2 vessel plane section). These results suggest that mild hypothermia such as winter swimming may be a potential tool for preventing cancer among people with pre-malignant tumors or who are otherwise at high risk for cancer. This study may shed new light on the relative contributions of cancer prevention by thermophysical method using mild hypothermia.
Yonggang Lv Xiaoying Hao Li Yang Manping Lin
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, P.R.China Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing 400044, P.R.China
国际会议
成都
英文
1-3
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）