Molecular and phenotypic characterization of transgenic tobacco expressing the Arabidopsis IRT1 gene
In order to enhance the iron accumulation in plants, the primers were designed based on the Arabidopsis thaliana IRT1 sequence in GenBank. The total RNA of Arabidopsis thaliana roots was extracted after iron deficiency treatment for 24h. The cDNA of IRT1 gene was cloned by RT-PCR and then was inserted into the pBluescript II SK+vector. After confirmed by endonuclease digestion assay and gene sequencing, the IRT1 gene was subcloned into the Sac I and BamH I targeting sites of the vector pBI121, and the plant expression vector pBI121IRT1 was constructed. The pBI121IRT1 vector was transformed into the wild type tobacco by Agrobacterium mediated method. Seventy-six resistant shoots were obtained by Kanamycin selection. 21 were identified by PCR and PCR-Southern detection. Five transformants were selected to perform the physiological assay respectively. The results showed the leaf chlorophyll contents were higher in the transformants than in the control, while the leaf MDA contents were lower in the transformants. It indicated that the IRT1 gene enhanced the iron uptake in the transformants and improved the transformants′ resistance to iron deficiency.
Li Liya Cai Qiuyi Jia Kai Zhang Meng Guo Changhong
Key Laboratory of Molecular and Cytogenetics, Heilongjiang Province； Department of Biology, Harbin N Key Laboratory of Molecular and Cytogenetics, Heilongjiang Province； Department of Biology, Harbin N
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）