Determination of genomic sequence of Lily symptomless virus
cDNA containing the 3′ part sequences of seven Lily symptomless virus (LSV) isolates were obtained by RT-PCR and sequenced. The sequenced region is corresponding to partial CP gene and the entire sequences of 16kDa gene located at the 3′ end of LSV genome. The sequence homologies of partial CP and 16 kDa protein genes, compared with other reported isolates, were 87.4% to100% and 97.9% to 100% respectively. At the amino acid level, they shared 95.9% to 100% and 95.7% to 100% sequence similarity respectively. A RNA slide hybridization assay was used to detect the natural occurrence of LSV in lily plants, from both imported cultivars and wild plants. The occurrence frequency of LSV was 40.0% in lily samples provided by Beijing Enter& Exit Inspection and Quarantine Bureau; and it was 56% and 60% in the samples from Lishui and Haining, Zhejiang Province respectively; the frequency of LSV was 40.0% in the samples of lily varieties from Kunming, Yunnan Province and Guangzhu, Guangdong Province respectively. Thus, LSV infection occurs at a relatively high frequency in field lily from China.
Weiguo Chai Chong Wang Lijuan Han Shaoning Chen Yuchuang Zhang Jishuang Chen
Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou, China Hangzhou Academy of Agric Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou, China Beijing Entry & Exit Inspection and Quarantine Bureau, Beijing, China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）