Studies on the Physiological Response of Brassica chinensis L. Seeds in Germination to Environmental Pollutant LAS Toxicity by FTIR-ATR Spectroscopy
(0) A technique based on Fourier transform infrared attenuated total reflection (FTIR-ATR)spectrometry was used to study the physiological changes in chemical composition of Brassica chinensis L. Seeds under a wide range of the alkylbenzene sulphonates(LAS) treatments.The results showed that,after 3d of LAS stress, the absorption bands corresponding to carbohydrates,ester,proteins and protein secondary structure varied differently. The absorption band peaks at 1 632 cm -1 、1 742cm -1 which are significantly higher than that of control group (P<0.05), suggesting the proteins and ester content increased. Fourier self-deconvolution (FSD) analysis result showed that LAS caused the relative ratio of the protein conformation forms as well as α -helix,β -turn and random coil have change. The intensities of carbohydrates absorption bands of radicle and seedling shows different change trends, that shows LAS has different influence on carbohydrate metabolism in radicle and seedling. Furthermore, the band height of FTIR of radicle showed a correlation to the germination percentage, radicle lenth,inhibition rate of radicle length, Superoxide Dismutase (SOD) activity, Malondialdehyde (MDA) concent. As a result , FTIR-ATR technique can be acceptd as rapid and simple way to study the physiologcal mechanism of plant for adapting the stres of LAS.
Yan-hong Bai Ning Gong Xiao-nong Zhao Gong-ming Yang
College of Food and Biotechnology Engineering ,Zhengzhou University of Light Industry,Zhengzhou 4500 College of Science,Northwest A & F University,Yangling 712100,China College of Food Science,South China Agricultural University,Guangzhou 510642,China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）