Neurotoxicity Evaluation of Chlorpyrifos Exposure with Caenorhabditis elegans
Chlorpyrifos, a broad spectrum orgnaophosphorus insecticide, is extensively used in agricultural and household insect control, but the potential health effects associated human exposure to low levels is unclear, which has been subject of increasing concern in the last years. However, few studies about chlorpyrifos toxicity have been conducted in the model organism Caenorhabditis elegans, which can complement both in vitro and in vivo mammalian models in toxicology. In the present study, Caenorhabditis elegans was chosen to evaluate the neurotoxicity of chlopyrifos with 0.003, 0.03, 0.3, and 3 mg/L after 4h exposure by analyzing basic movements, feeding ability and inhibition of acetylcholinesterase activity. Results indicated that forward turn frequency and acetylcholinesterase activity were reduced significantly compared with control at the highest concentration group, while feeding ability showed to decrease significantly at the lowest concentration group. The impact of feeding ability seems more sensitive than other targets. Moreover, we assessed the postexposure recovery of basic movements after the 4h exposure of Chlorpyrifos. Forward turn frequence of worms exposed to the highest concentration exposure failed to recovery, so that this suggested chlorpyrifos may result in the long term health effect at high concentration exposure for Caenorhabditis elegan. Results also demonstrated that Caenorhabditis elegans could be a good model for chlorpyrifos-induced toxic effects research.
Jing Juan Ju Qin Li Ruan Yun Hui Li Ran Liu Li Hong Yin Yue Pu Pu
School of Public Health, Southeast University Key Laboratory of Environmental Medicine and Engineering, Ministry of Education Nanjing, China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）