Impact of Estradiol Benzoate and Progesterone on the Activity of Carbonic Anhydrase of Erythrocytes in Ducks (Anatis domesticae Caro)
The impact of estradiol benzoate and progesterone on the duck erythrocyte carbonic anhydrase (CA) activity of in vitro culture was studied. The impact of estradiol benzoate and progesterone on CA activity of in vitro culture duck erythrocytes was significant and varied with the incubation durations. When the duck erythrocytes were incubated 2, 14 hours, estradiol benzoate increased the CA activity. When the duck erythrocytes were incubated thirty hours, estradiol benzoate at a higher addition (more than 200 pg ml -1) had no facilitation to the CA activity. Progesterone with addition in the range of 0.5 to 50 ng ml -1 promoted the CA activity when the duck erythrocytes were incubated fourteen hours. The CA activity was also decreased by progesterone when the duck erythrocytes were incubated two hours in MEM medium containing progesterone at the addition of 1 to 10 ng ml -1. Progesterone at the addition of 0.5 ng ml -1 inhibited the CA activity when the duck erythrocytes were incubated thirty hours. These findings can help to understand the interaction between CA and reproductive hormones, and account for the impact of exogenous hormones administration or endogenous estrogens analogs inhalation on the eggshell formation of right thickness and some other biological functions.
Y.Y.Wu X.Z.Zhao P.P.Li Y.Y.Wu
Key Lab of Modern Agri.Equip.&Technol., Ministry of Education & Jiangsu Province, Institute of Agri. State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry Chinese Academy of Sci
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）