Oxidative Stress-mediated Pro-inflammatory Responses in Lung Epithelial Cells Exposed to Multi-walled Carbon Nanotubes
Reactive oxygen species (ROS) and activation of redox-sensitive transcription factors such as nuclear factor (NF)-κ B have been implicated in pro-inflammatory responses induced by nanoparticles. However, there is still only little knowledge about the pro-inflammatory responses induced by multi-walled carbon nanotubes (MWCNTs). The objective of the present study was to compare the ability of MWCNTs and carbon black (CB) nanoparticles to induce secretion of pro-inflammatory cytokines and to explore possible mechanisms in A549 lung epithelial cells. Exposure of A549 cells to MWCNTs, but not to CB, resulted in a significant increase in interleukin-8 (IL-8) release and gene expression. MWCNTs stimulated NF-κ B DNA binding activity, nuclear translocation of the p65 subunit of NF-κ B, IκBα.phosphorylation, and ROS generation in A549 cells. MWCNTs-induced IL-8 gene expression and NF-κB activation were abrogated in the presence of ROS scavengers. These results suggest that MWCNTs are able to induce pro-inflammatory responses and NF-κB activation in A549 cells mediated by ROS generation.
Min Han Shefang Ye Wen Wen Qiqing Zhang
Research Center of Biomedical Engineering, Department of Biomaterials, College of Materials, Xiamen Research Center of Biomedical Engineering, Department of Biomaterials, College of Materials, Xiamen
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）