The toxicity evaluation of Gentamicin and Lincomycin injection on pig kidney PK-15 cells

摘要：

To explore the toxicity of the Gentamicin andLincomycin injection, PK-15 cells were treated with (0.2-3.2mg/mL) for 24-72h. Its effect of growth inhibition on cells was measured by 3-(4,5dimethyl-thiazol)-2,5-diphenyltetrazoliumbromide (MTT). The determination of leakage of LDH and NAG was performed using commercial kit. The results showed that the Gentamicin and Lincomycin injection inhibited the growth of PK-15 cells, and moreover, the inhibition increased linearly with the increase of the injection concentrations and times of incubation, Namely, as its concentration increased, LDH or NAG leakage also increased. It is concluded that the Gentamicin and Lincomycin injection exerted certain toxicity on cells.

作者: Haiyan JIA Qianqian XU Jichang LI Yanhua LI

作者单位: College of Veterinary Medicine Northeast Agricultural University Harbin, ChinaLincomycin injection, PK-15 cells were treated with 0.2-3.2mg/mL for 24-72h.Its effect of growth inhibitionon cells was measured by 3-4,5dimethyl-thiazol-2,5-diphenyltetrazoli

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）