HPLC Determination chlorogenic acid in Do-Zhong (Eucommia ulmoides) leaves by ultrasolic extraction

摘要：

In order to analysis of chlorogenic acid in Do-Zhong(Eucommia ulmoides), ultrasolic extraction was used to prepare the sample. With yield of chlorogenic acid as index, The effect of ethanol concentration, ultrasolic power. extraction time, and the ratio of solid to liquid were studied. The extracts were then analysis by high-performance liquid chromatographic (HPLC) method. The results shown that the optimum extraction conditions were as follows: 70% ethanol(w/w) , extraction power 200W, extraction time 18 min, stock ratio 1:10(g:mL). For HPLC analysis, the calibration plot was linear (R 2=0.9993) in the concentration range 200 ～1000 μ g/mL for chlorogenic acid,The method was precise with relative standard deviation for chlorogenic acid range between 1.50 ～2.55%. The recovery of this method were between98.46 -100.05%.The method could be used as a practical technique for detect chlorogenic acid in Do-Zhong for it was samples rapid, simple, sensitive, convenient..

作者: Xueyuan Jin Hong Liu

作者单位: Department of Chemistry Engineering Huainan Union University Huainan,China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）