Synthesis and Identification of Artificial Antigen for Bensulfuron-methyl

摘要：

In this study, artificial antigens for Bensulfuron-methyl (BSM) were prepared by means of two-step procedure of glutaraldehyde method. The hapten BSM was covalently attached to ovalbumin to synthesis immunogen (BSM-OVA), and hapten Benzyl sulfonamide (BS) was linked to BSA to prepare coating antigen (BS-BSA). The conjugates were identified by UV spectrophotometry and SDS-PAGE. After immunized with BSM-OVA to BALB/c mice, high titer and sensitive polyclonal antibodies against bensulfuron-methyl herbicide were obtained. The titer was 1 : 1.28×10 5. The IC50 was 0.032 μ g/mL. The results indicated that the artificial antigen for BSM synthesized successfully, and it could be used to produce monoclonal antibodies for BSM.

作者: Yu Xiao-wei Wei Chen-xi Xia Ying Zhang Ya-ran Yuan Jun-lin

作者单位: Hubei Key Laboratory of Genetic Regulation and Integrative Biology College of Life Science, Huazhong Normal University Wuhan 430079, China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）