Dtermination of Trace Oxytetracycline in Wasterwater by Phase-separation Floatation with Ultraviolet Detection

摘要：

In the present work, a novel analytical method was proposed for the determination of trace oxytetracycline(OTC) in wastewater by phase-separation floatation(PSF) coupled with an ultraviolet detector setting at 375 nm. OTC can be transformed into tenary complex(Co-OTC-DNA) in the presence of cobalt(Ⅱ) and deoxyribonucleic acid (DNA) which can be separated by phase-separation floatation easily. The optimum phase-separation floatation was performed on phase-separation reagent using 1 mol·L-1 sodium chloride, caustic soda solution as acidity regulator and tetrahydrofuran(THF) as solvent. The linear regression equation is A=7.85×105C-0.002, linear range is from 5.0×10-8-1.0×10-5 mol·L-1, lower limit of detection (LOD) was 3.82×10-9 mol·L-1. The recoveries of OTC in water samples were 99.1-99.4%.

作者: HOU Yan-mina CHENG Yong-hua YAN Yong-sheng

作者单位: School of chemistry and chemical engineering,pingdingshan university,Henna Pingdingshan,467000,China School of chemistry and chemical engineering,jiangsu university,Zhenjiang,212013,China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）