Determination of Substituted Benzenes in Water by Salting-out Phase Separation Microextraction Coupled to High Performance Liquid Chromatography
A trace analytical method of o-chloropheno, chlorobenzene and ethylbenzene in water had been developed by using salting-out phase separation microextraction coupled to high performance liquid chromatography. A Diamonsil C18 column (5μm, 250×4.6 mm i.d.) was used for separation at 30℃ with detection wavelength at 220 nm; The mobile phase was a mixture of methanol and water (the ratio of volume was 53:47), flow rate was 1.0 mL/min. The optimal extraction conditions: volume of water was 18mL; extraction solvent was 300μL iso-butyl alcohol; quantity of ammonium sulfate was 14g. Good linearity was verified in a range of 0.006-138.4 mg/L for each analyte (r2>0.9999). LODs of o-chlorophenol, chlorobenzene and ethylbenzene were 0.07, 0.2, 6.4μg/L, respectively. The average recovery of substituted benzenes ranged from 71.66% to 92.96% with the relative standard deviations (RSDs) (n=6) below 5.17%.
Jian-ke ZHOU Kun LONG Jing PENG
Research Center of Physics and Chemistry Analysis, Hebei University, Key Laboratory of Medical Chemistry and Molecular Diagnosis, Ministry of Education Baoding, 071002, China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）