Biodegradation of Higher Concentration Phenol by Pseudomonas aeruginosa HS-D38
Phenol is an important and common environmental pollutant. In this study, Pseudomonas aeruginosa HS-D38 strain was used to degrade phenol, and the degradation characteristics were investigated. Results indicated that HS-D38 strain could degrade phenol and utilize it as the sole carbon, nitrogen and energy source. UV-visible spectra analysis showed that 300 mgL-1 phenols could be completely degraded within 10 h by HS-D38 strain after an initial lag phase of 2 h, and the peak decreased gradually with the degradation of phenol by HS-D38. At the same time, an increase of OD600 values in the culture was also observed. Phenol could be mineralized at higher concentration of 500 mgL-1 by HS-D38 strain, whereas the degradation did not occur when the concentration was exceeded 700 mgL-1 even after 72 h. A key intermediate was detected analyzing the degradation solution with HPLC and it was identified to p-hydroxybenzoic acid. The peaks disappeared after 15 h indicating intermediates were also degraded by HS-D38 strain and the metabolism proceeded through the p-hydroxybenzoic acid pathway.
Yongliang Zheng Jianping Gan Jun Xiang Yuanping Fang Deli Liu Jianping Fang
College of Life Science and Engineering, Huanggang Normal University, 146 Xingang II Road, Huangzhou College of Life Science and Engineering,Huanggang Normal University, 146 Xingang II Road, Huangzhou College of Life Sciences, Central China Normal University, 152 Luoyu Road, Wuhan 430079, Hubei Provi
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）