Dynamics of Nitrobacteria Community in Biological Contact Oxidation Process by DGGE and FISH
The performance and nitrobacter population dynamics of a pilot-scale biological contact oxidation process which was used to purify a landscape river, were evaluated during the biofilm forming period over 45 days. Ammonia oxidizing bacteria (AOB) community structure and composition of nitrobacteria in the system were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and fluorescence in situ hybridization (FISH) techniques. The results showed that 30% average removal efficiency of NH4+-N and 15% average removal efficiency of chemical oxygen demanded (CODMn) were achieved. DGGE analysis represented a dynamic population with gradually evolved dominant species. The number of superior bands increased with the development of biofilm, and Data from 16S rRNA sequencing demonstrated genera Nitrosomonas was the dominant AOB in this system. FISH results suggested that AOB and nitrite-oxidizing bacteria (NOB) accounted for high up to 9.9% and 3.8% of the total bacteria, respectively, and Nitrospira was the dominant members of NOB in the biofilm. The general nitrobacteria population coupled with the performance of biological contact oxidation process indicated that the dynamics of the community structure of nitrobacteria was important indicator to evaluate the effect of the biofilter system.
Shuying He Jing Chen Jixiang Li Yatong Xu Haizhen Yang
College of Environmental Science and Engineering School of Economics and Management Tongji University Shanghai, China College of Environmental Science and Engineering Tongji University Shanghai, China Shanghai Key Laboratory of Urbanization and Ecological Restoration East China Normal University Shan College of Environmental Science and Engineering Tongji University
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）