Enzymatic esterification of ammonium lactate with ethanol in organic solvent: kinetic study

摘要：

The kinetics of the esterification of ammonium lactate with ethanol catalyzed by Novozym 435 was investigated. The Ping-Pong Bi-Bi enzymatic mechanism with competitive inhibition by both substrates was proposed. The corresponding kinetic parameters were calculated by non-linear regression. rmax=75.6 μmol/(g·min). The inhibition constants for lactic acid (A) and ethanol (B) were KiA=0.016 mol/L and KiB=0.38 mol/L, respectively. The model was found to be in good agreement with the experimental data. As we know, it is the first time for the kinetic study of the ethyl lactate synthesis, which used ammonium lactate as raw material and catalyzed by immobilized lipase.

作者: Yingying Wang Yanjun Jiang Liya Zhou Jing Gao

作者单位: Department of Bioengineering Hebei University of Technology Tianjin, P.R.China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）