Gene delivery with nanofibrous scaffolds
Extracellular and intracellular barriers typically prevent non-viral gene vectors from having an effective transfection efficiency. Formulation of a gene delivery vehicle that can overcome the barriers is a key step for successful tissue regeneration. We have developed a novel core-shelled DNA nanoparticle by invoking solvent-induced condensation of plasmid DNA (β -galactosidase or GFP) in a solvent mixture (94% DMF+6% 1xTE buffer) and subsequent encapsulation of the condensed DNA globule in a triblock copolymer, polylactide-poly(ethylene glycol)-polylactide (L8E78L8), in the same solvent environment. The polylactide shell protects the encapsulated DNA from degradation during electrospinning of a mixture of encapsulated DNA nanoparticles and biodegradable PLGA (a random copolymer of lactide and glycolide) to form a nanofibrous non-woven scaffold using the same solution mixture. The bioactive plasmid DNA can then be released in an intact form from the scaffold with a controlled release rate and transfect cells in vitro.
CHU Benjamin LIANG Dehai HSIAO Benjamin S.
Chemistry Department, Stony Brook University, Stony Brook, New York 11794-3400 Department of Polymer Science & Engineering, College of Chemistry & Molecular Engineering,Peking Uni
国际会议
2009 International Conference on Advanced Fibers and Polymer Materials(2009年先进纤维与聚合物材料国际学术会议)
上海
英文
554-558
2009-10-21（万方平台首次上网日期，不代表论文的发表时间）