Immobilization of cellulase on chitosan-coated magnetic nanoparticles modified with α-ketoglutaric acid
Enzyme immobilization technology has recently attracted lots of attention, and the key step in immobilization processes lies in the appropriate carrier materials, which confers numerous benefits, such as facile recovery of the enzyme and high activities of immobilized enzymes, etc. In this study, cellulase was first covalently immobilized to a magnetic polymer nanoparticles, which is chitosan-coated magnetic nanoparticle modified with α-ketoglutaric acid (α-KA-CCMNPs). Cellulase was immobilized to α-KA-CCMNPs by activating its carboxyl groups with carbodiimide, and forming O-acylisourea that can be readily attached to the proteins amino groups. Under optimum condition, the immobilized cellulase obtained a high relative activity of 94.3%. The maximum activity of immobilized cellulase occurred at pH 5.0 and the enzyme concentration of 12 mg/ml. Comparing with the chitosan-coated magnetic nanoparticles (CCMNPs), the activity of cellulase immobilized on α-KA-CCMNPs was higher than that on CCMNPs. These results indicate that the α-KA-CCMNPs are a promising immobilization support for large-scale preparation of immobilized cellulase for industrial application.
ZHOU Yuting NIE Huali SONG Mingmin ZHU Limin
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University,Shanghai 201620, P.R.China
国际会议
2009 International Conference on Advanced Fibers and Polymer Materials(2009年先进纤维与聚合物材料国际学术会议)
上海
英文
1103-1105
2009-10-21（万方平台首次上网日期，不代表论文的发表时间）