Expression of Rhizopus oryzae Lipase for Biodiesel Production
The Rhizopus oryzae lipase containing prosequence (ProROL) was successfully expressed in pichia pastoris. The SDS-PAGE analysis of the gene product showed a subunit molecular mass of 32 kD, which is consistent with the molecular mass of Rhizopus oryzae lipase opened on GeneBank. The maximum activity of recombinant lipase obtained from Muts KM71H/pPICZαA-ProROL recombinant was 90 IU/ml in shaking flask fermentation level. The enzyme was stable in broad ranges of temperatures and pH, with the optimal temperature at 35 ℃ and pH 7.0. Metal ions such as K+, Mg2+, Ba2+and Zn2+gently activated the enzyme activity, but the enzyme was sensitve to organic solution. The recombinant Rhizopus oryzae lipase could be well used for the transesterification of plant oils even at higher water content up to 60～100％ w/w based on oil mass, and the yield of methyl ester could reach over 95％ in the transesterification system. The results indicate that the recombinant lipase is an effective biocatalyst for enzymatic biodiesel production.
Rhizopus oryzae Lipase Expression Characterization Pichia pastoris Biodiesel
Zhi-lin LI Xun LI Ye WANG You-dong WANG Fei WANG Jian-chun JIANG
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, PR China College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, PR China Institute of Institute of Chemistry and Industry of Forest Products, Nanjing 210042, PR China
国际会议
南京
英文
126-132
2010-10-22（万方平台首次上网日期，不代表论文的发表时间）