Production of Biodiesel Fuel from Pistacia chinensis bge Seed Oil Using Immobilized Lipase
Enzymatic transesterification of Pistacia chinensis bge seed oil (PCO) with methanol has been studied. PCO was extracted from P. chinensis seeds with petroleum ether, and its yield was 33.4％. Using a recombinant Rhizopus oryzae lipase (ROL) immobilized on macroporous resin as biocatalyst, the effects of water content, molar ratio of alcohol to oil, enzyme loading, enzyme stability, and temperature on the transesterification reaction were investigated. The optimum conditions for the transesterification of PCO with methanol were as follows: dosage of the immobilized recombinant ROL at 12％(w/w) based on oil mass (or 6.9 U/g PCO), water content 10～40％ (w/w) based on oil mass, molar ratio of methanol to oil 5:1, reaction temperature 37℃, and reaction time 60 h. Under the conditions, the maximum yield of methyl esters (ME) reached 94％. Furthermore, the reusability of the biocatalyst was also investigated under the same conditions. The yield of ME could be remained above 60％ after the immobilized biocatalyst was repeatedly used for 4 times.
Pistacia chinensis bge oil immobilized enzyme biodiesel Rhizopus oryzae lipase.
Xun LI Chun-yu WANG You-dong WANG Fei WANG Jian-chun JIANG
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China Institute of Chemistry and Industry of Forest Products, CAF, Nanjing 210037, China
国际会议
南京
英文
172-179
2010-10-22（万方平台首次上网日期，不代表论文的发表时间）