High Consistency Enzymatic Hydrolysis of Steam-Exploded Corn Stover
To improve economical efficiency of the lignocellulosic materials conversion to ethanol, three-stage hydrolysis of steam-exploded corn stover at high substrate concentration and the reuse of β-glucosidases were investigated. During the three-stage hydrolysis, cellulosic substrate was hydrolyzed for 9, 9, and 12 h, respectively, with a total hydrolysis time of 30 h. The three-stage (9+9+12 h) hydrolysis could reach much higher hydrolysis yields at 70.41％, and increase about 36％ than conventional hydrolysis process for 72 h. The results indicated that short-time hydrolysis and the removal of end products at each stage significantly benefited the adsorption of cellulase enzyme to the solid substrate. The maximum hydrolysis yield at substrate concentration of 25％ reached at 85.1％ in 30 h when steam-exploded corn stover was used as the substrate for cellulase synthesis. Furthermore, the supernatant of three-stage hydrolysis, including glucose and cellobiose, was hydrolyzed using β-glucosidase. and the ultrafiltration technique was employed to recover the β-glucosidase. The result showed that the β-glucosidase remained relatively active after eight rounds of recycling.
high substrate concentration three-stage hydrolysis cellulase corn stover
Jing YANG Xiao-ping ZHANG Qiang YONG Shi-yuan YU
Southwest Forestry University, Kunming, China, 650224 Key Laboratory of Forest Genetics & Biotechnol Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry Universit
国际会议
南京
英文
222-231
2010-10-22（万方平台首次上网日期，不代表论文的发表时间）