Effects of Ion Implantation on Cellulase Producing Strain Trichoderma reesei
(0) Trichoderma reesei was used as experimental model, and the effects of low-energy N+implantation on its survival rate, total anti-oxidation capability (T-AOC), protein concentration and cellulase yield were investigated. The results showed that the dose-survival rate curve took “double saddle shape with increasing N+implantation doses. It was different from the typical “saddle shape curve of ion beam implantation. While the T-AOC activity-dose curve also has two peaks at the dose of 1.50×1016 ions·cm-2 and 2.50×1016 ions·cm-2, it was exactly the same as its survival rate curve. So we speculated that the changes of T-AOC activities probably decided the microbe survival situation after being implanted by low-energy N+. Three cellulase high yielding strains T.reesei 150-1, 150-2 and 250-6 were obtained and the average production of cellulase are raised by 24.3％ in shaking flasks. Comparing with the control strain, the mutants were potent in accumulating cellulase especially in the later stage.
Trichoderma reesei cellulose N+ implantation “double saddle shape curve
Ning ZHANG Jian-chun JIANG Wei-di DAI Jian ZHAO
Institute of Chemical Industry of Forest Products, CAF Key and Open Lab On Forest Chemical Engineering, SFA. Nanjing 210000, China
国际会议
南京
英文
367-371
2010-10-22（万方平台首次上网日期，不代表论文的发表时间）