The Chemical Conposition and Extraction Process of Ginkgo Protein
Abstract: Kjeldahl method was used to measure the protein content from ginkgo seeds in different area,i.e.Jiangsu, Hubei and Guangxi in china, and in different species,i.e., Longyan ginkgo,Dafuzhi ginkgo, round-shape ginkgo, potato-shape ginkgo, plum-shape ginkgo, bergamot ginkgo. Composition of ginkgo protein and its extraction process were also studied in this paper.The results showsed that protein contents in different species were at 7.86％～11.88％, moisture at 56.01％～60.01％, the relative protein contents in five extract parts,i.e.,water-soluble, salt-soluble, alcohol-soluble，alkali-soluble and insoluble,were 22.20％, 17.99％, 1.73％, 30.32％ and 25.97％ respectively. The highest extract yield of salt-soluble protein was 99.516％ by ultrasound-assisted extraction at the optimal conditions of 0.14mol/L NaCl solution as extraction agent,PH8, solid-liquid ratio 1:7 (m/v), extraction 30min, 3 times. By using DEAE-cellulose, we can get salt-soluble protein at a purity of 90.77％, which was further treated by HCl for analyzing on changes of amino acids by HPLC,the result demonstrated Lysine climbed from 1.25％ to 4.53％, Serine raised from 1.83％ to 10.55％, and Histidine 10.34％ to 44.24％.
ginkgo protein amino acids Kjeldahl HPLC DEAE-cellulose
Xi-juan CHEN Cheng-zhang WANG Hong-xia CHEN Jiang-zhong YE
Institute of Chemical Industry of Forest Products, CAF, Key and Open Lab.on Forest Chemical Engineering,SFA,Nanjing 210042 ,China
国际会议
南京
英文
772-779
2010-10-22（万方平台首次上网日期，不代表论文的发表时间）