PRODUCTION OF ENZYMES OF BIOTECHNOLOGICAL/ENVIRONMENTAL INTEREST FROM BANANA PEEL
In this work, the agricultural residues, banana peel, were selected as potential substrates for ligninolytic enzyme production by White Rot Fungi. These microorganisms are known for their capacity of hydrolysing complex organic compound. Banana peel (BP) is an abundant waste that has high content in carbohydrates and trace elements, allowing its application in biotechnological processes. A factorial design of experiments was utilized to study the influence of nitrogen source, organic and inorganic, in the production of ligninolytic enzymes using banana peel as carbon source. Additionally, the ion Mn+2 was added to culture medium as inductor of Mnoxidizing peroxidase activity. Statistical analysis indicates that the combination of lowest N level (0.2 g L-1) and highest Mn level (150 mM) was the best condition to produce Mn-oxidizing peroxidase. Laccase and aryl-alcohol oxidase were also detected in the cultures but not lignine peroxidase. The cultures with peptone instead of ammonium tartrate increased two-fold the levels of this activity. These results suggest that banana peel could be used as nutrient source to grow white rot fungi and to produce their ligninolytic enzymes.
Banana peel White rot fungi Ligninolytic enzymes MnP
C. PALMA M.J. MARTíNEZ I.E. GROSSMANN M. DOMACH
Universidad de Santiago de Chile, Santiago, Chile Centro de Investigaciones Biológicas, CSIC, Madrid, Spain Carnegie Mellon University, Pittsburgh, USA
国际会议
北京
英文
919-924
2010-05-17（万方平台首次上网日期，不代表论文的发表时间）