A FACILE METHOD FOR MEASURING INITIAL ADSORPTION OF CELLULASE ON CELLULOSE SUBSTRATE

摘要：

A facile method based on flow-measurement and UV-visible detection was established for measuring the kinetic adsorption of cellulase on cellulose in the initial stage. A calibration curve relating absorbance at 280 nm and that at 500 nm caused by fine fibers was set up for diminishing the interference of insoluble particles. A rapid and a slow phase can be observed during the steady-state adsorption process and can be described by intraparticle diffusion and pseudo-second-order models, respectively. The method is applicable to pure cellulose and thoroughly washed lignocellulose substrates, spectacularly at low concentration (<2.5％).

关键词： initial adsorption cellulase kinetics flow-measurement adsorption models

作者: Qin Weiwei Fu Shiyu Liu Hao Chai Xinsheng Zhan Huaiyu

作者单位: State Key Laboratory of Pulp and Paper Engineering,South China University of Technology, Guangzhou,510640, China

会议类型: 国际会议

会议名称: 4th ISETPP(第四届制浆造纸新技术国际研讨会)

会议地点: 广州

会议语种:英文

页码: 169-172

在线出版日期: 2010-11-08（万方平台首次上网日期，不代表论文的发表时间）