CHANGE OF STRUCTURE AND CHARACTERISTICS OF LIGNIN DURING DILUTED SULFURIC ACID PRETREATMENT OF CORN STOVER AND ADSORPTION OF CELLULASE TO LIGNIN
In this study, milled wood lignin (MWL) was extracted from diluted sulfuric acid pretreated- and raw corn stover, and structure and characteristics of lignin were studied to investigate the changes of lignin in acid pretreatment, and adsorption of cellulase to lignin was researched to evaluate the effect of lignin on cellulase complex. GPC analysis showed that average molecular weight of MWL from pretreated corn stover was greater than that of the un-pretreated sample, and their dispersity was 1.00 and 1.34 respectively. 1H-NMR and 13C-NMR spectrum showed thatβ-1, β-5, β-O-4 substructures decreased during the pretreatment while β-β almost kept constant. Different dosages of MWL were added to the same enzyme reaction system to investigate the effect of MWL on adsorption of enzyme cellulase. It was showed that protein content and FPA reduced dramatically during the first 12 hours, and almost kept constant from 12 to 72 hours. The reduction of protein content and FPA were proportional to lignin dosage. However, lignin dosage had little influence on activity of β- glucosidase during 72 hours. To decrease adsorption of cellulase to lignin, some surfactants were screened. It showed that PEG-8000 was best for decreasing the adsorption of cellulase to lignin and improving the enzymatic hydrolysis of lignocellulosic materials.
lignin diluted sulfuric acid pretreatment corn stover cellulose adsorption
Yao Lan Yang Haitao Li Xuezhi Yue Jun Zhao Jian Qu Yinbo
State Key Laboratory of Microbial Technology,Shandong University, 250100, China Key Laboratory of Paper Science and Technology of Ministry of Education, Shandong Institute of Light
国际会议
广州
英文
421-424
2010-11-08（万方平台首次上网日期，不代表论文的发表时间）