COMPARATIVE THERMAL STABILITY AND ADSORPTION ON VARIOUS BLEACHED FIBER SOURCES OF A RECOMBINANT VOLVARIELLA VOLVACEA ENDOGLUCANASE I (EG1) WITH TS TRUNCATED CATALYTIC CORE (EG1-CD)
Recombinant Volvariella volvacea endoglucanase I (EG1) and its catalytic module (EG1-CD) were purified by two-step chromatography, and the thermal stability and adsorption on bleached fibers were compared. The results indicated that the thermal stability of EG1-CD decreased 17％~28％ within the temperature range of 30~60℃ compared with that of EG1, and at lower temperature, a greater gap of thermal stability between EG1 and EG1-CD was found. The binding capacities of EG1 and EG1-CD onto various fiber sources such as bleached wheat straw AP-AQ pulp (WSBAP), hardwood kraft pulp (LBKP), softwood kraft pulp (NBKP) and chemi-thermomechanical pulp(CTMP) were 85％~58％ and 45％~25％, respectively. The binding capacities of EG1 and EG1-CD onto various fractions of bleached softwood fiber were found different as well.
endoglucanase(EG) adsorption cellulose binding domain (CBD)
Wu Shufang Ding Shaojun Li Zhongzheng
Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing,210037, China
国际会议
广州
英文
689-692
2010-11-08（万方平台首次上网日期，不代表论文的发表时间）