OPTIMIZING SPORL PRETREATMENT FOR ROBUST SUGAR PRODUCTION FROM ASPEN
This study investigated the sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) for robust sugar production from aspen. Aspen wood chips were pretreated with sulfur acid (0~2.2％, on oven dry wood, the same in the following) and sodium bisulfite 0~4.5％) at 160~180oC for 0～30 min, and then refined using a disk refiner to produce lignocellulosic substrates. The resultant substrates were enzymatically saccharified using 7.5 FPU cellulase plus 11.25 IU β-glucosidase per gram of od substrate. The hemicellulosic sugars were directly recovered from the pretreatment spent liquor. It was found that about 95％ of the Xylan and 50％ of the lignin were removed by SPORL at 170oC for 20 min with sulfur acid and sodium bisulfite charge of 1.1 and 3％, respectively. About 95％ of the glucan retained in the solid substrate was converted to glucose by the enzymes. It was also found that the inhibitors in pretreatment liquor were reduced with the addition of sodium bisulfite compared with dilute acid pretreatment under the same acid dosage. SPORL pretreatment significantly reduced the energy consumption in post-pretreatment wood size reduction to about 24 wh/kg od untreated wood. The total monomeric sugar recovered from SPORL pretreatment is about 90％ of the theoretical value, never achieved by competing technologies.
SPORL enzymatic saccharification size reduction aspen cellulosic ethanol
Zhu Wenyuan Zhu Junyong Chen Kefu
Jiangsu Provincial Key Lab of Pulp and Paper,Science and Technology, Nanjing Forestry University,Nan USDA Forest Service, Forest Products Laboratory,Madison, WI, USA Key Laboratory of Pulp and Paper Engineering,South China University of Technology, Guangzhou,510640,
国际会议
广州
英文
1320-1323
2010-11-08（万方平台首次上网日期，不代表论文的发表时间）