IMPROVING ENZYMATIC HYDROLYSIS OF POPULUS TOMENTOSA CARR. USING DIFFERENT CELLULOSE-DISSOLUTION PRETREATMENTS
This study aims to establish an efficient pretreatment process using cellulose-dissolution solvents to enhance the enzymatic saccharification by cellulase. Three cellulose dissolution agents, LiOH/urea solution, concentrated phosphoric acid, and ionic liquid (1-butyl-3-methylimidazolium chloride; BMIMCl), were selected to dissolve the cellulosic preparation from Populus tomentosa Carr. In comparison with conventional cellulose pretreatment processes, the dissolution pretreatments were operated under a milder condition with temperature <130 °C and ambient pressure. When it was subjected to the solution and rapid precipitation with water, the cellulosic preparation exhibited lower cellulose crystallinity and degree of polymerization (DP). Pretreated materials were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy and chemical methods. The regenerated cellulosic preparations exhibited a significant enhancement on enzymatic hydrolysis kinetic. It was found that not only the glucose releasing rate but also hydrolysis yield was enhanced at least twofold as compared with that of unpretreated material. After 72 h, the saccharification yield ranged from 80％ to 92％ for the regenerated materials while only around 33％ could be achieved for the unpretreated cellulose preparation. In comparison, ionic liquid offered unique advantages in the hydrolysis rate in the first 12 hours probably due to the extensive destruction of the crystalline region. However, even with relatively higher crystallinity, cellulose regenerated from concentrated phosphoric acid dissolution achieved the highest saccharification yield.
enzymatic hydrolysis Populus Tomentosa Carr. pretreatment
Wang Kun Yang Haiyan Xu Feng Sun Runcang
Institute of Biomass Chemistry and Technology,Beijing Forestry University, Beijing, 100083, China Institute of Biomass Chemistry and Technology,Beijing Forestry University, Beijing, 100083, China St
国际会议
广州
英文
1340-1343
2010-11-08（万方平台首次上网日期，不代表论文的发表时间）