Studying circulation times of liver cancer cells by in vivo flow cytometry
Hepatocellular carcinoma (HCC) may metastasize to lung, bones, kidney, and many other organs. The survival rate is almost zero for metastatic HCC patients. Molecular mechanisms of HCC metastasis need to be understood better and new therapies must be developed. A recently developed in vivo flow cytometer combined with real-time confocal fluorescence imaging are used to assess spreading and the circulation kinetics of liver tumor cells. The in vivo flow cytometer has the capability to detect and quantify continuously the number and flow characteristics of fluorescently labeled cells in vivo in real time without extracting blood sample. We have measured the depletion kinetics of two related human HCC cell lines, high-metastatic HCCLM3 cells and low-metastatic HepG2 cells, which were from the same origin and obtained by repetitive screenings in mice. >60％ HCCLM3 cells are depleted within the first hour. Interestingly, the low-metastatic HepG2 cells possess noticeably slower depletion kinetics. In comparison, <40％ HepG2 cells are depleted within the first hour. The differences in depletion kinetics might provide insights into early metastasis processes.
G Liu Y Li Z Fan J Guo X Tan X Wei
Institutes of Biomedical Sciences, Fudan University, 138 Yi Xue Yuan Road,Shanghai, China 200032
国际会议
3rd International Photonics & OptoElectronics Meetings(第三届国际光子与光电子学会议 POEM 2010)
武汉
英文
1-8
2010-11-03（万方平台首次上网日期，不代表论文的发表时间）