Characterizing lamina propria of human gastric mucosa by multiphoton microscopy
Lamina propria (LP) of gastric mucosa plays an important role in progression of gastric cancer because of the site at where inflammatory reactions occur. Multiphoton imaging has been recently employed for microscopic examination of intact tissue. In this paper, using multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), high resolution multiphoton microscopic images of lamina propria (LP) are obtained in normal human gastric mucosa at excitation wavelength λτx = 800 nm. The main source of tissue TPEF originated from the cells of gastric glands, and loose connective tissue, collagen, produced SHG signals. Our results demonstrated that MPM can be effective for characterizing the microstructure of LP in human gastric mucosa. The findings will be helpful for diagnosing and staging early gastric cancer in the clinics.
Y C Liu H Q Yang G Chen S M Zhuo J X Chen J Yan
Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Te Department of Pathology, Fujian Provincial Tumor Hospital, Fuzhou, 350014,China Department of Surgery, Fujian Provincial Tumor Hospital, Fuzhou, 350014, China
国际会议
武汉
英文
1-4
2010-11-02（万方平台首次上网日期，不代表论文的发表时间）