Improved electroporation parameters of delivering silver nanoparticles into living C666 cells for surface-enhanced Raman scattering
Electroporation assisted metallic nanoparticle delivery has been shown by our previous work to significantly reduce the time of sample preparation for surface-enhanced Raman spectroscopy (SERS) measurements of biological cells, In this research note, we report our experimental work to optimize the electroporation parameters, including adjustment of the pulse pattern and operation temperature, for fastest delivery of silver nanoparticles into living C666 cells (a human nasopharyngeal carcinoma cell line). The delivery efficiency was evaluated by the integrated intensity of whole cell SERS spectrum. Our work concluded that the silver nanoparticle delivery rate is best under the electroporation condition of using 4 consecutive 350 V (875 V/cm) rectangular electric pulses of 1 ms, 10 ms, 10 ms, and 1 ms durations respectively. Low temperature (0~4°C) is necessary for improving the delivery efficiency of silver nanoparticles.
Yun Yu Juqiang Lin Zufang Huang Gangqin Xi Duo Lin Yongjian Chen Rong Chen Haishan Zeng
Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Key Laboratory of Optoelectronic Science and Technology for Medicine, Mnistry of Education, Fujian N Cancer Imaging Department, British Columbia Cancer Research entre,Vancouver, B.C., V5Z 1L3, Canada
国际会议
武汉
英文
1-5
2010-11-02（万方平台首次上网日期，不代表论文的发表时间）