A New Theoretical Approach to Single-Molecule Fluorescence Optical Studies of RNA Dynamics
Single-molecule fluorescence spectroscopy in condensed phases has many important chemical and biological applications. The single-molecule fluorescence measurements contain information about conformational dynamics on a vast range of time scales. Based on the data analysis protocols methodology proposed by X. Sunney Xie, the theoretical study here mainly focuses on the singlemolecule studies of single RNA with interconversions among different conformational states, to with a single FRET pair attached. We obtain analytical expressions for fluorescence lifetime correlation functions that relate changes in fluorescence lifetime to the distance-dependent FRET mechanism within the context of the Smoluchowski diffusion model. The present work establishes useful guideline for the single-molecule studies of biomolecules to reveal the complicated folding dynamics of single RNA molecules at nanometer scale.
Xinghai Zhao Guangcun Shan Shuying Bao
Institute of Electronic Engineering, China Academy of Engineering Physics,Mianyang 621900, China Institute of Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing 210003, State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and
国际会议
武汉
英文
1-8
2010-11-02（万方平台首次上网日期，不代表论文的发表时间）