Characteristics of spore-bound laccase from Bacillus subtilis WD23 and its use in dye decolorization
Treatment of xenobiotic compounds such as textile dyes with bacterial laccases is limited to the acid pH range and moderate temperatures.A bacterial strain,designated as WD23,was isolated from forest soil using Luria-Bertani medium supplemented with 0.4 mmol/L Cu2+.The isolated strain was identified as Bacillus subtilis by physiological and biochemical tests and 16S rDNA sequence analysis.Here we charactered the spore-bound laccase of B.subtilis WD23 and used the laccase to decolorize dyes.The spores of the strain showed laccase-like activity,oxidizing syringaldazine,2,6-dimethoxyphenol and 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonate acid)(ABTS).The optimum pH and temperature for the spore-bound laccase were 6.8 and 60℃,respectively.It also showed higher stabilities over a broad pH range,the pH half-life was more than 6 months at pH 6.8.The spore laccase could efficiently decolorize 50～90％ of anthraquinone and azo dyes in 24 h.The spore laccase can play an important role in bioremediation.
Bacterial laccase Spore Characteristics Bacillus subtilis Dye decolorization
ChunLei Wang Min Zhao XingDong Wei TaiLun Li Lei Lu
College of Life Sciences,Northeast Forestry University,Harbin,150040,China
国际会议
哈尔滨
英文
226-230
2010-07-24（万方平台首次上网日期，不代表论文的发表时间）