Extraction and Isolation of 7S and 11S from Low-Temperature Defatted Soybean Draff

摘要：

A semi-works production method which the extraction and isolation of 7S and 11S was improved.The method of isoelectric point and cooling precipitation was developed for extraction and isolation of 7S and 11S fractions from low-temperature defatted soybean draff,based on the indices of the extraction yield which was determined by the Kjeldahl method and purity which was determined by SDS-PAGE.Compared the optimum parameters of five kinds of extraction methods that phosphate buffers effect is better than others,its extraction yield is 89.55％.The optimal isolation parameters of 7S are as follows: pH 4.7,sedimentation time 1h,the stirring rate of acid 40r/min,its purity and yield are 73.25％ and 50.6％ respectively.The optimal isolation parameters of 11S are as follows: pH 6.2,cooling precipitation 12h,temperature 4℃,the stirring rate of acid 30r/min,its purity and yield were 76.37％ and 41.2％ respectively.With the improvements,the extraction yield of 11S and 7S fractions were significantly increased.They may be widely used as a source of highly functional soybean protein in more food products.

关键词： low-temperature defatted soybean draff 7S 11S extraction and isolation methods

作者: Shi Yanguo Yang Chunhua Yiu Ying Zhang Yifang Sun Bingyu Hu Chunlin

作者单位: Harbin University of Commerce ,Harbin 150076,China Harbin High-Tech Soybean Food Co.Ltd,.Harbin 150078,China

会议类型: 国际会议

会议名称: 2010 International Conference of Environment Materials and Environment Management(2010年环境材料与环境管理国际学术会议 EMEM 2010)

会议地点: 哈尔滨

会议语种:英文

页码: 403-406

在线出版日期: 2010-07-24（万方平台首次上网日期，不代表论文的发表时间）