Microwave Assisted Enzymatic Hydrolysis of Corn Stalks for L-lactic Acid Production
Glucose production from pretreated corn stalks by cellulose enzymatic hydrolysis with cellulase was investigated and compared with no pretreatment,as a reference.The corn stalks were pretreated with microwave,sulfuric acid hydrolysis and dilute sodium hydroxide solution hydrolysis respectively.The enzymatic hydrolysis experiments were carried out at 50℃,50 g/1 dry matter (DM) solid substrate concentration and 15 filter paper unit (IU)/g DM of a commercial cellulase.Fermentable sugar was able to be produced from all the pretreated corn stalks with an overall yield of 29-58％ of the maximum theoretical yield,based on the glucan available in the solid and liquid substrate.The corn stalks pretreated with dilute acid had the best glucose yield as 58.09％ followed by the corn stalks pretreated with acid and microwave with an overall yield of 57.02％ with 15 IU/g DM of cellulase.Glucose was the main product with enzymatic hydrolysis yield ratio 38.89％in the dilute sulfuric acid pretreated corn stalks,while with enzymatic hydrolysis yield ratio 51.07％in the dilute sodium hydroxide solution and microwave pretreated corn stalks under enzymatic hydrolysis conditions.These advantages,along with their negative price,make these solids a valuable raw material for L-lactic acid production.
microwave corn stalks cellulase enzymatic hydrolysis L-lactic acid
YANG Xue-xin FANG Gui-zhen
Key laboratory of Bio-based Material Science and Technology,Northeast Forestry University,Harbin,Chi Key laboratory of Bio-based Material Science and Technology,Northeast Forestry University,Harbin,Chi
国际会议
哈尔滨
英文
712-715
2010-07-24（万方平台首次上网日期，不代表论文的发表时间）