Study on Inter-relationship of the Nutrients in Substrate and the Production of Demulsifying Compound by a Bacillus sp.
This work investigated the effect of substrates on de-emulsification capability and the growth of a bacillus sp.(screened by our laboratory).The biomass and de-emulsification ratios(DR) of cultures grown in substrate with the different nutrients (glucose,liquid paraffin,NH4NO3 and yeast) were determined.In the orthogonal tests,the significant of de-emulsification capability in turn is glucose>liquid paraffm>yeast>NH4NO3.The glucose is the highest effect of nutrients on de-emulsification capability and growth.The de-emulsification ratios(DR) and the glucose utilization ratios increase simultaneously at log and lag growth phase.Measurements of the liquid paraffin residue indicates that 30.1％ liquid paraffin in substrate is consumed and used in inducing the demulsifying compounds production rather than the increase of the biomass.The culture grown in substrate with 2％～5％ liquid paraffin exhibits highest de-emulsification ratio(DR).The contents of yeast at 0.8mg/L～l.0mg/L is beneficial to the growth and demulsifying compound production.
biological demulsifier bacillus sp emulsion nutrient
Xu LI Ji-xian YANG Fang MA Chang LIU Jin-na WANG Ning HOU Yang XU
State Key Lab of Urban Water Resource and Environment,School of Municipal and Environmental Engineer School of Resources and Environment,Northeast Agriculture University,Harbin 150090,China
国际会议
哈尔滨
英文
974-979
2010-07-24（万方平台首次上网日期，不代表论文的发表时间）