The Molecular Characterization and Hydrogen Production of a New Species of Anaerobe in Wastewater Treatment Process for H2 Recovery
The isolation and identification of hydrogen production bacteria(HPB)with high yield and high evolution rate is an important foundation of fermented hydrogen production process by anaerobic digesting high strength organic wastewater.An improved Hungater rolling tubes technique and a plate method of culture bottle (PMCB) were employed to enumerate and isolate the anaerobes.The HPB-RL medium was designed specially for isolating and culturing anaerobic hydrogen bacteria.Culture temperature was at 37℃ and 5.5 of pH.About 90 strains of bacteria were isolated,in which,a strain of the hydrogen producer with high yield and high evolution rate was isolated and named Rennanqil2.The isolates were straight rods,Grams positive,strictly anaerobic,no spore-forming bacteria,whole bacteria flagella,having capsule,3～4 metachromatic granules.The strain of Rennanqi12 could produce hydrogen using molasses wastewater as the carbon resource.The isolate Rennanqil2 has the closest relationship with the strain C.cellulose for the 94％ homology of 16S rDNA.The strain of Rennanqi 12 might be a new species that belongs to a new genus.The amount of hydrogen-producing (YH2)is 1880.5ml/L medium and the maximum hydrogen-producing rate(QH2)is 28.2 mmol/g·(dry·cell)·h when R12 digested substrates were glucose.
Keyword Hydrogen production anaerobe 16S rDNA sequencing H2-produicng capacity
Liu Xiaoye Guo Zirui Zhang Yongjuan Wang Zhanqing Li Yongfeng
School of Forestry ,Northeast Forestry University,Harbin,150040,China School of Forestry ,Northeast Forestry University,Harbin,150040,China College of Chemistry and Chemi
国际会议
哈尔滨
英文
1049-1052
2010-07-24（万方平台首次上网日期，不代表论文的发表时间）