Cloning of Salt-Resistance Transcription Factor Gene and Identification of Function in Soybean Plants
In order to verify the transcription factor CBF2 gene can be induced to express in plant and improve the plant salt resistance,they were cloned from the Arabidopsis thaliana by PCR according to CBF2 gene sequence published on GenBank.The plant expression vector (pBI-rd29A-CBF2) with CBF2 gene was constructed.The CBF2 gene was transformed into soybean plants via Agrobacterium-medialed transformation.It approved that the CBF2 gene had been transformed and integrated into the genome of soybean by PCR and Southern blot.Through the physiological test of salt resistance,the relative electric conductivity of the transformed plants plasma membrane and the control were 36.84％ and 59.35％ respectively under salt stress.The content of chlorophyll of the transformed plants was 2.41 times as much as that of the control.The content of proline of the transformed plants was 2.49 times as much as that of the control.It showed that the CBF2 gene was induced to express in soybean plants.And it also indicated that the expression of the CBF2 gene increased the salt stress tolerance of the transgenic soybean plants.The results proved the CBF2 gene were functional gene.
Soybean CBF2 gene clone expression salt-resistance
Qu Min Zhang Yanming Li Xinling Wang Quanwei
Harbin University of Commerce,Harbin,150076,China Harbin Normal University,Harbin,150080,China
国际会议
哈尔滨
英文
1324-1327
2010-07-24（万方平台首次上网日期，不代表论文的发表时间）