Culture medium optimization for photosynthetic bacteria
Photosynthetic bacteria (PSB) have various applications but the culture cost is very expensive.To find an efficient and economic culture medium the effects of yeast extract,C/N ratio,and trace elements on the growth of PSB were studied.The results showed that the optimal condition for PSB growth was: yeast extract of 100mg/l,C/N of 12:1,and trace elements (in mol/L): Mn2+ (0.009),Fe3+ (0.0025),Co2+ (0.0024),Cu2+ (0.0024),and Zn2+ (0.0033).Trace element lack could affect the growth of PSB.The order was Mn2 +> Fe3 +> Co2+> Cu2 +> Zn2 +.The improved medium was named HCH,and the optimum medium components were (in g/1) DL-malic acid: (4),MgSO4: (0.12),(NH4)2SO4: (1),CaCl2: (0.075),KH2PO4: (0.5),K2HPO4: (0.3),Na2EDTA: (0.02),yeast extract: (0.1),trace elements lml (in mol/1 ): Fe3+: (0.0025),Mn2+: (0.009),Zn2+: (0.0033),Co2+: (0.0024),pH:6.8.Comparing with the traditional RCVBN medium,in HCH medium yield of PSB increased 1.2 times and the cost decreased 19.8 times.
PSB Culture medium Optimization
Wei Zhao Chunhua He Guangming Zhang
School of Municipal and Environmental Engineering,Harbin Institute of Technology,73 Huanghe Road,Har Jiangshu Transportation Research Institute co.,LTD,223 Shuiximen Road,Nanjing,210017,China
国际会议
哈尔滨
英文
1443-1446
2010-07-24（万方平台首次上网日期，不代表论文的发表时间）