TOPOCHEMICAL DISTRIBUTION OF LIGNIN AND CELLULOSE IN POPULUS NIGRA DETERMINED BY MICRO-RAMAN SPECTROSCOPY

摘要：

Topochemical distribution of lignin and cellulose in individual cell wall layers of Populus nigra stem was determined by transmission electron microscopy (TEM) and confocal Raman microscopy. TEM images exhibited that the fiber wall is typically differentiated into three layers: middle lamella (ML), primary wall (P) and secondary wall (S1, S2 and S3), and the staining intensities represent differing lignin concentrations. In situ Raman images calculated by integrating over the intensity of characteristic spectral bands enabled visualization of the spatial variation in lignin and cellulose. Raman images acquired by integrating over spectral band at 1605 cm-1 suggested that higher lignin concentration was visualized in the cell corner (CC), the compound middle lamella (CML) and the secondary wall of ray parenchyma. Cellulose distribution followed by taking the band regions around 2897 cm-1 into account showed the opposite pattern with the highest concentration in fiber secondary wall.

关键词： populus nigra cell wall polymers TEM imaging raman imaging

作者: J. F. Ma Z.H.Zhang Z. ao F.Xu

作者单位: Institute of Biomass Chemistry and Technology, Beijing Forestry University, Beijing 100083, China Institute of Biomass Chemistry and Technology, Beijing Forestry University, Beijing 100083,China Institute of Biomass Chemistry and Technology, Beijing Forestry University, Beijing 100083, China.

会议类型: 国际会议

会议名称: 16th International Symposium on Wood,Fiber and Pulping Chemistry(第十六届木材、纤维及制浆化学国际会议)

会议地点: 天津

会议语种:英文

页码: 621-625

在线出版日期: 2011-06-08（万方平台首次上网日期，不代表论文的发表时间）