EFFECTS OF WET PRESSING-INDUCED FIBER HORNIFICATION ON ENZYMATIC SACCHARIFICATION OF LIGNOCELLULOSES
This study reported the effect of wet pressinginduced fiber hornification on enzymatic saccharification of lignocelluloses. A wet cellulosic substrate of bleached kraft eucalyptus pulp and two wet sulfite pretreated lignocellulosic substrates of aspen and lodgepole pine were pressed to various moisture (solids) contents by variation of pressing pressure and pressing duration. Wet pressing reduced substrate moisture content and produced irreversible reduction in fiber pore volume - fiber hornification - as reflected from the reduced water retention values (WRV), an easily measurable parameter, of the pressed substrates. The wet-pressing resulted in a reduction in substrate enzymatic digestibility (SED). It was found that the reduction in SED was approximately 20％ for the two sulfite pretreated substrates when moisture content was reduced from approximately 75％ to 35％. The reduction for the cellulosic substrate was less than 10％ when its moisture content was reduced from approximately 65％ to 35％. The results indicated that the reduction in SED is negligible and observable when samples were pressed to solids content to 40％ and 50％, respectively. Furthermore, a less digestible sample is more susceptible to wet-pressing to reduce SED. It was also found that WRV can correlate to SED of homified substrates resulted from the same never dried or pressed sample independent of the bonification process, e.g., pressing or drying. This correlation can be fitted using a Boltzman function. Cellulase adsorption measurements indicated that wetpressing induced fiber hornification reduced cellulose accessibility to cellulose, which is the only factor to reduce SED. The results obtained in this study provide guidelines to high solids enzymatic saccharification of pretreated biomass.
high solids enzymatic hydrolysis/saccharification fiber hornification wet pressing cellulose accessibility water retention value (WRV)
X. L. Luo J. Y. Zhu R. Gleisner H.Y.Zhan
State Key Lab Pulp and Paper Engineering, South China University of Technology, Guangzhou, China USD USD A Forest Service, Forest Products Laboratory,Madison, WI, USA Dept. of Biological Systems Engine USD A Forest Service, Forest Products Laboratory,Madison, WI, USA State Key Lab Pulp and Paper Engineering, South China University of Technology, Guangzhou, China
国际会议
16th International Symposium on Wood,Fiber and Pulping Chemistry(第十六届木材、纤维及制浆化学国际会议)
天津
英文
922-927
2011-06-08（万方平台首次上网日期，不代表论文的发表时间）