FACTORS IMPACTING ETHANOL PRODUCTION BY RECOMBINANT ZYMOMONAS MOBILIS
Xylose-metabolising recombinant Z mobilis was constructed in order to effectively utilize lignocelluloses to produce fuel ethanol by cofermentaion of glucose and xylose. However, cofermentation efficiency was less than that of glucose fermentation. However, co-fermentation efficiency of xylose and glucose was less than that of glucose fermentation. Therefore, this study was carried out to investigate the influence of internal and external factors on cofermentation. Three recombinant strains with different terminator of foreign genes were constructed, and they shared similar xylose fermentation performance. The effect of environmental conditions on co-fermentation was evaluated. The optimum conditions for co-fermentation of glucose and xylose were temperature 30~34 °C and pH5.5. Ethanol productivity of co-fermentation in the pH range 4.5~7.5 or temperature range 26~40°C depended on efficiency of xylose metabolism. However, when die stirring speed was 50-400 r/min, the ethanol productivity remained unchanged. The results comprehensively indicated the factors impacting ethanol production using recombinant Z mobilis. Hence, this investigation could provide a valuable clue for harnessing the biomass resource effectively.
recombinant Zymomonas mobilis co-fermentation ethanol production
Yuanyuan Ma Shaolan Zou Jiefang Hone Huina Dong Yang Liu Minhua Zhang
Tianjin University R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, Chin Tianjin University R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, Chin
国际会议
16th International Symposium on Wood,Fiber and Pulping Chemistry(第十六届木材、纤维及制浆化学国际会议)
天津
英文
1023-1027
2011-06-08（万方平台首次上网日期，不代表论文的发表时间）