GROWTH AND LIGNOLYTIC ENZYMES PRODUCTION DURING CULTIVATION OF WHITE ROT FUNGI IN ACIDIC SULFITE SPENT LIQUOR OF BAGASSE
In view of current economical and environmental problems in recent years, integral utilization of sugarcane bagasse, an energy crop residue, has been a high priority for the diversification of the sugarcane industry. In this paper, the fungal biomass and laccase production in pretreatment spent liquor of bagasse using three strains of white rot fungi, Panus conchatus, Flammulina velutipes and Psathyrella candollean, was investigated. The performance of three pretreatment methods, a mixture of 5％ NaHSO3 and 0.2 ％ H2SO4 (A), 5 ％ NaHSO3 (B) and 0.2％ H2SO4 (C), was compared for fermentation at 160℃ for 15 min with a 1:8 solid-to-liquor ratio. The majority of the monosaccharide in spent liquor from the pretreatment methods A, B and C was xylose, with the concentrations of 11.3, 3.7 and 10.6 g/L, respectively. The biomass of all the three strains of white rot fungi increased during fermentation in the three kinds of liquid medium based on the spent liquor of bagasse without detoxification, but only Flammulina velutipes showed excellent enzyme production with the maximal laccase activity of about 200, 180 and 130 U/L in the medium, respectively. It should be noted that our results demonstrated the potential for laccase production from pretreatment spent liquor of bagasse by white rot fungi.
bagasse pretreatment white rot fungi biomass laccase
Kai Fu Shiyu Fu Hao Liu Huaiyu Zhan
State Key Laboratory of Pulp and Paper Engineering,South China University of Technology, Guangdong 510640,China
国际会议
16th International Symposium on Wood,Fiber and Pulping Chemistry(第十六届木材、纤维及制浆化学国际会议)
天津
英文
1172-1175
2011-06-08（万方平台首次上网日期，不代表论文的发表时间）